foxp1 antibody  (ABclonal Biotechnology)

Journal: Genes & Development

Article Title: Independent control of neurogenesis and dorsoventral patterning by NKX2-2

doi: 10.1101/gad.352886.125

Figure Lengend Snippet: In vitro generation of limb-level mouse motor neurons. ( A ) Schematic of ventral progenitor domains in the brachial (caudal cervical) spinal cord; at this level, p3 progenitors generate V3 interneurons (INs) instead of branchiovisceral motor neurons. ( B ) Summary of culture conditions that produce brachial motor neurons. ( C ) Immunofluorescence images of day 7 mouse cultures show that HOXC8 + somatic motor neurons (MNX1 + /ISL1 + ) are produced, some of which are FOXP1 + , indicating LMC-like identity. ( D ) Immunofluorescence images of day 5 cultures show that motor neurons (ISL1 + ) produced under these conditions are somatic (MNX1 + ). No expression of PHOX2A is observed. ( E ) RT-qPCR for Sim1 mRNA shows a >1000-fold expression increase in late stages of culture. ( F ) Summary of proportions of cells expressing NKX2-2, ISL1, and OLIG2 (normalized to the total number of cells expressing at least one of these three genes) over time.

Article Snippet: Primary antibodies, host species, and concentrations used in this study were as follows: ISL1 (goat, 1:5000, has 10% cross-reactivity to ISL2; Neuromics GT15051-100; RRID: AB_2126323), MNX1 (guinea pig, 1:100; from Jessell Laboratory), FOXP1 (mouse, 1:400; Santa Cruz Biotechnology sc-398811), NKX2-2 (mouse, 1:100; Developmental Studies Hybridoma Bank [DSHB] 74.5A5; RRID: AB_531794), BrdU (rat, 1:400; Abcam ab6326; RRID: AB_305426), OLIG2 (guinea pig, 1:100; from Jessell Laboratory), IRX3 (rabbit, 1:100; from Jessell Laboratory), and PAX6 (mouse, 1:100; DSHB supernatant).

Techniques: In Vitro, Immunofluorescence, Produced, Expressing, Quantitative RT-PCR

Journal: Clinical and Translational Medicine

Article Title: T‐cell differentiation stage block bias confers hypermethylation and mediastinal preference in T‐cell lymphoblastic lymphoma

doi: 10.1002/ctm2.70380

Figure Lengend Snippet: UHRF1 affects the malignancy of T‐cell lymphoblastic lymphoma (T‐LBL) through DNA methylation. (A) Scatter plot indicating differentially methylated loci (DMLs) in Uhrf1‐sufficient and Uhrf1‐deficient T cells, with red dots representing the DMLs. (B) Scatter plot displaying DEGs in Uhrf1‐sufficient and Uhrf1‐deficient T cells, with red dots representing the DEGs. (C) Metascape network enrichment analysis of intersected DMLs and DEGs, displaying the top 20 enriched terms; dot colours indicate different enriched terms. (D) Scatter plot indicating differentially methylated regions (DMRs) in vector‐ and UHRF1‐knockdown cells, with red dots representing the DMRs. (E) Scatter plot indicating DEGs in vector‐ and UHRF1‐knockdown cells, with red dots representing the DEGs. (F) Heatmap illustrating the Pearson correlation between target downstream genes and UHRF1 in the scT‐LBLs. (G) Methylation profile of the target genes of UHRF1 in vector control and UHRF1‐knockdown cells, namely, FOXP1 , PIK3R1 and KLF6 . H. Verification of target proteins of UHRF1 by WB analysis in vector‐ and UHRF1‐knockdown cells. FOXP1, PIK3R1 and KLF6 were significantly upregulated following UHRF1 knockdown. The data are presented as the means ± SDs, and p values were calculated by two‐tailed t ‐test.

Article Snippet: For mouse tumour slides, we examined changes in target protein expression following demethylation treatment with the following antibodies: FOXP1 (1:200, A23442, ABclonal), PIK3R1 (1:200, 60225‐1‐Ig, Proteintech) and KLF6 (1:200, A10011, ABclonal).

Techniques: DNA Methylation Assay, Methylation, Plasmid Preparation, Knockdown, Control, Two Tailed Test

Journal: bioRxiv

Article Title: Temporally-segregated dual functions for Gfi1 in the development of retinal direction-selectivity

doi: 10.1101/2025.06.03.657700

Figure Lengend Snippet: Gfi1 is expressed in direction-selective D-oDSGC and F-mini ON RGC subsets ( A ) Schematic depicting Gfi1 expression in the retina restricted to D-oDSGCs, F-mini-ONs and W3Bs. D-oDSGCs and F-mini ONs are direction-selective subsets tuned primarily to downward motion, while W3Bs are non-direction-selective. ( B ) Wholemount P6 Gfi1 Cre , LSL-YFP retina shows YFP expression restricted to Rbpms + RGCs. ( C ) Density recovery profile (DRP) of YFP + RGCs suggests an absence of mosaicism confirming Gfi1 expression in more than one RGC subtype. ( D ) Density of Gfi1-expressing RGCs in the retina. ( E ) In situ hybridization in P4 retina cryosection shows Gfi1 expression in Fibcd1 + D-oDSGCs. ( E’, E” ) Detailed views of the nuclei indicated in D. ( F ) Quantification of the percentage of Gfi1 + RGCs co-expressing Fibcd1 . ( G ) Coronal brain section from Gfi1 Cre , LSL-tdTomato adult mouse shows strong innervation of the ventral medial terminal nucleus (MTN) by tdTomato + RGCs. ( H-N ) Detailed views of all retinorecipient nuclei labeled with cholera toxin B (CTB) from Gfi1 Cre , LSL-YFP adult mice confirms YFP + RGC innervation exclusively in the ventral MTN (H, H’ ) and the SC ( I, I’ ), and not in the other retinorecipient nuclei ( J-N ). ( O ) Wholemount P1 Gfi1 Cre , LSL-YFP retina shows YFP expression in a subset of Foxp2 + RGCs. ( P, Q ) These YFP + Foxp2 + RGCs account for ∼25% of all Foxp2 + RGCs and ∼50% of all Gfi1 + RGCs. ( R ) Combinatorial labeling can differentiate between F-RGC subsets. ( S-U ) Wholemount Gfi1 Cre , LSL-YFP retina shows that YFP + Foxp2 + RGCs do not express the F-RGC OFF marker Foxp1 or the F-midi ON marker Brn3c, confirming their F-mini ON identity. Data are presented as mean ± SE.

Article Snippet: Primary antibodies used in this study include: Rabbit anti-dsRed (Living Colors, 1:1000), Chick anti-GFP (AVES, 1:1000), Guinea Pig anti-Foxp2 (Synaptic Systems, 1:500), Rabbit anti-Rbpms (Proteintech, 1:500), Guinea Pig anti-Rbpms (ThermoFisher, 1:500), Mouse anti-Brn3c (Santa Cruz, 1:100), Rabbit anti-Foxp1 (Proteintech, 1:500), Rabbit anti-IBA1 (Wako Laboratory Chemicals, 1:500), Goat anti-ChAT (Abcam, 1:250), Goat anti-VAChT (Millipore, 1:250) and Rabbit anti-cFos (Cell Signaling Technology, 1:500).

Techniques: Expressing, In Situ Hybridization, Labeling, Marker